Cleanroom Maintenance Significantly Reduces Abundance but Not Diversity of Indoor Microbiomes.

ABSTRACT: Cleanrooms have been considered microbially-reduced environments and are used to protect human health and industrial product assembly. However, recent analyses have deciphered a rather broad diversity of microbes in cleanrooms, whose origin as well as physiological status has not been fully understood. Here, we examined the input of intact microbial cells from a surrounding built environment into a spacecraft assembly cleanroom by applying a molecular viability assay based on propidium monoazide (PMA). The controlled cleanroom (CCR) was characterized by ~6.2*103 16S rRNA gene copies of intact bacterial cells per m2 floor surface, which only represented 1% of the total community that could be captured via molecular assays without viability marker. This was in contrast to the uncontrolled ad

SUBMITTER: Mahnert A 

PROVIDER: S-EPMC4537314 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature