Integrating microbial and host transcriptomics to characterize asthma-associated microbial communities.

ABSTRACT:

Background

The relationships between infections in early life and asthma are not completely understood. Likewise, the clinical relevance of microbial communities present in the respiratory tract is only partially known. A number of microbiome studies analyzing respiratory tract samples have found increased proportions of gamma-Proteobacteria including Haemophilus influenzae, Moraxella catarrhalis, and Firmicutes such as Streptococcus pneumoniae. The aim of this study was to present a new approach that combines RNA microbial identification with host gene expression to characterize and validate metagenomic taxonomic profiling in individuals with asthma.

Methods

Using whole metagenomic shotgun RNA sequencing, we characterized and compared the microbial communities of individual

SUBMITTER: Castro-Nallar E 

PROVIDER: S-EPMC4537781 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature