Impacts of K562 cells towards activities of Toll-like receptor pathway of human mesenchymal stem cell-bone marrow.

ABSTRACT: The study aim was to investigate the impacts of K562 cells towards the activities of Toll-like receptor pathway of human mesenchymal stem cell-bone marrow (HMSC-bm). The in vitro co-culture of HMSC-bm and K562 cells was set as the experiment group (HMSC-bm + K562), the HMSC-bm cultured alone was set as the control group (HMSC-bm), the expressions of six interested genes and their proteins, namely MyD88, P38, NF-κB, TAB1, TLR3 and TBK1, of the Toll -like receptor signaling pathway were detected and compared, as well as the secretions of such cytokines as IL-6, IL-8, TNF-α and IFN-α in the cell supernatant, which were regulated by the Toll-like receptor pathway. The expressions of MyD88, P38, TAB1 and TLR3 of the HMSC-bm + K562 group were higher than the HMSC-bm group, while that of TBK1 was

SUBMITTER: Xu Z 

PROVIDER: S-EPMC4537973 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature