Discovery of a drug targeting microenvironmental support for lymphoma cells by screening using patient-derived xenograft cells.

ABSTRACT: Cell lines have been used for drug discovery as useful models of cancers; however, they do not recapitulate cancers faithfully, especially in the points of rapid growth rate and microenvironment independency. Consequently, the majority of conventional anti-cancer drugs are less sensitive to slow growing cells and do not target microenvironmental support, although most primary cancer cells grow slower than cell lines and depend on microenvironmental support. Here, we developed a novel high throughput drug screening system using patient-derived xenograft (PDX) cells of lymphoma that maintained primary cancer cell phenotype more than cell lines. The library containing 2613 known pharmacologically active substance and off-patent drugs were screened by this system. We could find many compounds

SUBMITTER: Sugimoto K 

PROVIDER: S-EPMC4538400 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature