Rational design of antibodies targeting specific epitopes within intrinsically disordered proteins.

ABSTRACT: Antibodies are powerful tools in life sciences research, as well as in diagnostic and therapeutic applications, because of their ability to bind given molecules with high affinity and specificity. Using current methods, however, it is laborious and sometimes difficult to generate antibodies to target specific epitopes within a protein, in particular if these epitopes are not effective antigens. Here we present a method to rationally design antibodies to enable them to bind virtually any chosen disordered epitope in a protein. The procedure consists in the sequence-based design of one or more complementary peptides targeting a selected disordered epitope and the subsequent grafting of such peptides on an antibody scaffold. We illustrate the method by designing six single-domain antibodies t

SUBMITTER: Sormanni P 

PROVIDER: S-EPMC4538631 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature