Efficient method to optimize antibodies using avian leukosis virus display and eukaryotic cells.

ABSTRACT: Antibody-based therapeutics have now had success in the clinic. The affinity and specificity of the antibody for the target ligand determines the specificity of therapeutic delivery and off-target side effects. The discovery and optimization of high-affinity antibodies to important therapeutic targets could be significantly improved by the availability of a robust, eukaryotic display technology comparable to phage display that would overcome the protein translation limitations of microorganisms. The use of eukaryotic cells would improve the diversity of the displayed antibodies that can be screened and optimized as well as more seamlessly transition into a large-scale mammalian expression system for clinical production. In this study, we demonstrate that the replication and polypeptide dis

SUBMITTER: Yu C 

PROVIDER: S-EPMC4538638 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature