Structural basis for inhibition of the histone chaperone activity of SET/TAF-Iβ by cytochrome c.

ABSTRACT: Chromatin is pivotal for regulation of the DNA damage process insofar as it influences access to DNA and serves as a DNA repair docking site. Recent works identify histone chaperones as key regulators of damaged chromatin's transcriptional activity. However, understanding how chaperones are modulated during DNA damage response is still challenging. This study reveals that the histone chaperone SET/TAF-Iβ interacts with cytochrome c following DNA damage. Specifically, cytochrome c is shown to be translocated into cell nuclei upon induction of DNA damage, but not upon stimulation of the death receptor or stress-induced pathways. Cytochrome c was found to competitively hinder binding of SET/TAF-Iβ to core histones, thereby locking its histone-binding domains and inhibiting its nucleosome asse

SUBMITTER: Gonzalez-Arzola K 

PROVIDER: S-EPMC4538685 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature