Three-dimensional visualization of multiple synapses in thick sections using high-voltage electron microscopy in the rat spinal cord.

ABSTRACT: This data article contains complementary figure and movies (Supplementary Movies 1-3) related to the research article entitled, "Effective synaptome analysis of itch-mediating neurons in the spinal cord: a novel immunohistochemical methodology using high-voltage electron microscopy" [7]. It is important to show the synaptic connections at the ultrastructural level to understand the neural circuit, which requires the three-dimensional (3-D) analyses in the electron microscopy. Here, we applied a new sample preparation method, a high-contrast en bloc staining according to the protocol of the National Center for Microscopy and Imaging Research (NCMIR), University of California, San Diego, CA, USA to high-voltage electron microscopy (HVEM) tomography in order to examine the 3-D chemical neuroa

SUBMITTER: Satoh K 

PROVIDER: S-EPMC4539164 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature