Identification of a Three-Biomarker Panel in Urine for Early Detection of Pancreatic Adenocarcinoma.

ABSTRACT:

Purpose

Noninvasive biomarkers for early detection of pancreatic ductal adenocarcinoma (PDAC) are currently not available. Here, we aimed to identify a set of urine proteins able to distinguish patients with early-stage PDAC from healthy individuals.

Experimental design

Proteomes of 18 urine samples from healthy controls, chronic pancreatitis, and patients with PDAC (six/group) were assayed using GeLC/MS/MS analysis. The selected biomarkers were subsequently validated with ELISA assays using multiple logistic regression applied to a training dataset in a multicenter cohort comprising 488 urine samples.

Results

LYVE-1, REG1A, and TFF1 were selected as candidate biomarkers. When comparing PDAC (n = 192) with healthy (n = 87) urine specimens, the resulting areas under th

SUBMITTER: Radon TP 

PROVIDER: S-EPMC4539580 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature