Systemic modeling myeloma-osteoclast interactions under normoxic/hypoxic condition using a novel computational approach.

ABSTRACT: Interaction of myeloma cells with osteoclasts (OC) can enhance tumor cell expansion through activation of complex signaling transduction networks. Both cells reside in the bone marrow, a hypoxic niche. How OC-myeloma interaction in a hypoxic environment affects myeloma cell growth and their response to drug treatment is poorly understood. In this study, we i) cultured myeloma cells in the presence/absence of OCs under normoxia and hypoxia conditions and did protein profiling analysis using reverse phase protein array; ii) computationally developed an Integer Linear Programming approach to infer OC-mediated myeloma cell-specific signaling pathways under normoxic and hypoxic conditions. Our modeling analysis indicated that in the presence OCs, (1) cell growth-associated signaling pathways, P

SUBMITTER: Ji Z 

PROVIDER: S-EPMC4539608 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature