MicroRNA-132 promotes estradiol synthesis in ovarian granulosa cells via translational repression of Nurr1.

ABSTRACT:

Background

Estrogen synthesis is an important function of the mammalian ovary. Estrogen plays important roles in many biological processes, including follicular development, oocyte maturation and endometrial proliferation, and dysfunctions in estrogen synthesis contribute to the development of polycystic ovary syndrome and premature ovarian failure. Classical signaling cascades triggered by follicle-stimulating hormone induce estrogen synthesis via the upregulation of Cyp19a1 in granulosa cells (GCs). This study aimed to determine the effect of microRNA-132 (miR-132) on estradiol synthesis in GCs.

Methods

Primary mouse GCs were collected from ovaries of 21-day-old immature ICR mice through follicle puncture. GCs were cultured and treated with the stable cyclic adenosine mono

SUBMITTER: Wu S 

PROVIDER: S-EPMC4539686 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature