The cutaneous microbiome in outpatients presenting with acute skin abscesses.

ABSTRACT:

Background

Previous studies have demonstrated an association between antibiotic use and the development of skin abscesses. We tested the hypothesis that alterations in the composition of the cutaneous microbiota may predispose individuals to skin abscesses.

Methods

We studied 25 patients with skin abscesses and 25 age-matched controls, who each completed a questionnaire. Skin swab samples were obtained for DNA analysis from 4 sites around the abscess site (hereafter, "peri-abscess specimens") and from similar sites on the patient's contralateral side and on healthy control subjects. DNA was extracted and analyzed by quantitative polymerase chain reaction (qPCR) and high-throughput sequencing. The purulent abscess drainage was sent for culture.

Results

Fifteen patients

SUBMITTER: Horton JM 

PROVIDER: S-EPMC4539909 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature