Serine/arginine-rich splicing factor 3 (SRSF3) regulates homologous recombination-mediated DNA repair.

ABSTRACT:

Background

Our previous work found that serine/arginine-rich splicing factor 3 (SRSF3) was overexpressed in human ovarian cancer and the overexpression of SRSF3 was required for ovarian cancer cell growth and survival. The mechanism underlying the role of SRSF3 in ovarian cancer remains to be addressed.

Methods

We conducted microarray analysis to profile the gene expression and splicing in SRSF3-knockdown cells and employed quantitative PCR and western blotting to validate the profiling results. We used chromatin immunoprecipitation to study transcription and the direct repeat green fluorescent protein reporter assay to study homologous recombination-mediated DNA repair (HRR).

Results

We identified 687 genes with altered expression and 807 genes with altered splicing

SUBMITTER: He X 

PROVIDER: S-EPMC4539922 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature