To beat or not to beat a tick: comparison of DNA extraction methods for ticks (Ixodes scapularis).

ABSTRACT: Background. Blacklegged ticks (Ixodes scapularis) are important disease vectors in the United States, known to transmit a variety of pathogens to humans, including bacteria, protozoa, and viruses. Their importance as a disease vector necessitates reliable and comparable methods for extracting microbial DNA from ticks. Furthermore, to explore the population genetics or genomics of this tick, appropriate DNA extraction techniques are needed for both the vector and its microbes. Although a few studies have investigated different methods of DNA isolation from ticks, they are limited in the number and types of DNA extraction and lack species-specific quantification of DNA yield. Methods. Here we determined the most efficient and consistent method of DNA extraction from two different development

SUBMITTER: Ammazzalorso AD 

PROVIDER: S-EPMC4540005 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature