Identification and characterization of two ankyrin-B isoforms in mammalian heart.

ABSTRACT:

Aims

Excitation-contraction coupling in cardiomyocytes requires the proper targeting and retention of membrane proteins to unique domains by adaptor proteins like ankyrin-B. While ankyrin-B has been shown to interact with a variety of membrane and structural proteins located at different subcellular domains in cardiomyocytes, what regulates the specificity of ankyrin-B for particular interacting proteins remains elusive.

Methods and results

Here, we report the identification of two novel ankyrin-B isoforms AnkB-188 and AnkB-212 in human, rat, and mouse hearts. Novel cDNAs for both isoforms were isolated by long-range PCR of reverse-transcribed mRNA isolated from human ventricular tissue. The isoforms can be discriminated based on their function and subcellular distribution i

SUBMITTER: Wu HC 

PROVIDER: S-EPMC4540146 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature