TRPA1 and TRPV4 activation in human odontoblasts stimulates ATP release.

ABSTRACT: The mechanism of pain in dentine hypersensitivity is poorly understood but proposed to result from the activation of dental sensory neurons in response to dentinal fluid movements. Odontoblasts have been suggested to contribute to thermal and mechanosensation in the tooth via expression of transient receptor potential (TRP) channels. However, a mechanism by which odontoblasts could modulate neuronal activity has not been demonstrated. In this study, we investigated functional TRP channel expression in human odontoblast-like cells and measured ATP release in response to TRP channel activation. Human immortalized dental pulp cells were driven toward an odontoblast phenotype by culture in conditioned media. Functional expression of TRP channels was determined with reverse transcription polyme

SUBMITTER: Egbuniwe O 

PROVIDER: S-EPMC4541108 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature