Serum Dickkopf-1 as a Biomarker for the Diagnosis of Hepatocellular Carcinoma.

ABSTRACT:

Purpose

Dickkopf-1 (DKK-1) is a Wnt/β-catenin signaling pathway inhibitor. We investigated whether DKK-1 is related to progression in hepatocellular carcinoma (HCC) cells and HCC patients.

Materials and methods

In vitro reverse-transcription polymerase chain reaction (RT-PCR), wound healing assays, invasion assays, and ELISAs of patient serum samples were employed. The diagnostic accuracy of the serum DKK-1 ELISA was assessed using receiver operating characteristic (ROC) curves and area under ROC (AUC) analyses.

Results

RT-PCR showed high DKK-1 expression in Hep3B and low in 293 cells. Similarly, the secreted DKK-1 concentration in the culture media was high in Hep3B and low in 293 cells. Wound healing and invasion assays using 293, Huh7, and Hep3B cells showed that D

SUBMITTER: Kim SU 

PROVIDER: S-EPMC4541659 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature