DsRNA silencing of an R2R3-MYB transcription factor affects flower cell shape in a Dendrobium hybrid.

ABSTRACT:

Background

The R2R3-MYB genes regulate pigmentation and morphogenesis of flowers, including flower and cell shape, and therefore have importance in the development of new varieties of orchids. However, new variety development is limited by the long breeding time required in orchids. In this study, we identified a cDNA, DhMYB1, that is expressed during flower development in a hybrid orchid, Dendrobium hybrida (Dendrobium bobby messina X Dendrobium chao phraya) then used the direct application of dsRNA to observe the effect of gene silencing on flower phenotype and floral epidermal cell shape.

Results

Flower bud development in the Dendrobium hybrid was characterised into seven stages and the time of meiosis was determined as between stages 3 to 5 when the bud is approximately

SUBMITTER: Lau SE 

PROVIDER: S-EPMC4542095 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature