Development and evaluation of a SYBR Green real-time RT-PCR assay for detection of avian hepatitis E virus.

ABSTRACT:

Background

Avian hepatitis E virus (HEV) is the main causative agent of big liver and spleen disease, as well as hepatitis-splenomegaly syndrome in chickens. To date, conventional reverse transcriptase polymerase chain reaction (RT-PCR) and nested RT-PCR methods have been used for the diagnosis of avian HEV infection in chickens. However, these assays are time consuming, inconvenient, and cannot detect the virus quantitatively. In this study, a rapid and sensitive SYBR Green real-time RT-PCR assay was developed to detect avian HEV RNA quantitatively in serum, liver, spleen, and fecal samples from chickens.

Results

Based on the sequence of the most conserved HEV gene, ORF3, the primers for the assay were designed, and the standard plasmid was constructed. The detection limit

SUBMITTER: Zhao Q 

PROVIDER: S-EPMC4542097 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature