Ferric Uptake Regulator Fur Control of Putative Iron Acquisition Systems in Clostridium difficile.

ABSTRACT:

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Clostridium difficile is an anaerobic, Gram-positive, spore-forming opportunistic pathogen and is the most common cause of hospital-acquired infectious diarrhea. Although iron acquisition in the host is a key to survival of bacterial pathogens, high levels of intracellular iron can increase oxidative damage. Therefore, expression of iron acquisition mechanisms is tightly controlled by transcriptional regulators. We identified a C. difficile homologue of the master bacterial iron regulator Fur. Using targetron mutagenesis, we generated a fur insertion mutant of C. difficile. To identify the genes regulated by Fur in C. difficile, we used microarray analysis to compare transcriptional differences between the fur mutant and the wild type when grown in high-iron medium. The

SUBMITTER: Ho TD 

PROVIDER: S-EPMC4542176 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature