Heat-Labile Enterotoxin IIa, a Platform To Deliver Heterologous Proteins into Neurons.

ABSTRACT:

Unlabelled

Cholera toxin (CT) and the related heat-labile enterotoxins (LT) of Escherichia coli have been implicated as adjuvants in human therapies, but reactivity upon intranasal delivery dampened efforts to develop other clinical applications. However, each CT family member variant has unique biological properties that may warrant development as therapeutic platforms. In the current study, a nontoxic variant of the heat-labile enterotoxin IIa (LTIIa) was engineered to deliver heterologous, functional proteins into the cytosol of neurons. As proof of principle, the LTIIa variant delivered two cargos into neurons. LTIIa delivered β-lactamase efficiently into cells containing complex gangliosides, such as GD1b, as host receptors. LTIIa delivery of β-lactamase was sensitive to brefe

SUBMITTER: Chen C 

PROVIDER: S-EPMC4542185 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature