Unraveling dual feeding associated molecular complexity of salivary glands in the mosquito Anopheles culicifacies.

ABSTRACT: Mosquito salivary glands are well known to facilitate meal acquisition, however the fundamental question on how adult female salivary gland manages molecular responses during sugar versus blood meal uptake remains unanswered. To investigate these responses, we analyzed a total of 58.5 million raw reads generated from two independent RNAseq libraries of the salivary glands collected from 3-4 day-old sugar and blood fed Anopheles culicifacies mosquitoes. Comprehensive functional annotation analysis of 10,931 contigs unraveled that salivary glands may encode diverse nature of proteins in response to distinct physiological feeding status. Digital gene expression analysis and PCR validation indicated that first blood meal significantly alters the molecular architecture of the salivary glands. C

SUBMITTER: Sharma P 

PROVIDER: S-EPMC4542284 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature