An empirical review: Characteristics of plant microsatellite markers that confer higher levels of genetic variation.

ABSTRACT: During microsatellite marker development, researchers must choose from a pool of possible primer pairs to further test in their species of interest. In many cases, the goal is maximizing detectable levels of genetic variation. To guide researchers and determine which markers are associated with higher levels of genetic variation, we conducted a literature review based on 6782 genomic microsatellite markers published from 1997-2012. We examined relationships between heterozygosity (H e or H o) or allele number (A) with the following marker characteristics: repeat type, motif length, motif region, repeat frequency, and microsatellite size. Variation across taxonomic groups was also analyzed. There were significant differences between imperfect and perfect repeat types in A and H e. Dinucleot

SUBMITTER: Merritt BJ 

PROVIDER: S-EPMC4542939 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature