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Development of nuclear and chloroplast microsatellite markers for the endangered conifer Callitris sulcata (Cupressaceae).

ABSTRACT: PREMISE OF THE STUDY:Microsatellite markers were developed for Callitris sulcata (Cupressaceae), an endangered conifer species in New Caledonia. METHODS AND RESULTS:Using sequencing by synthesis (SBS) of an RNA-Seq library, 15 polymorphic nuclear and chloroplast microsatellite markers were developed. When evaluated with 48 individuals, these markers showed genetic variations ranging from two to 15 alleles and expected heterozygosity ranging from 0 to 0.881. CONCLUSIONS:These markers will be useful for examining the genetic diversity and structure of remaining wild populations and improving the genetic status of ex situ populations.

SUBMITTER: Sakaguchi S 

PROVIDER: S-EPMC4542945 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature

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Development of nuclear and chloroplast microsatellite markers for the endangered conifer Callitris sulcata (Cupressaceae).

Sakaguchi Shota S   Lannuzel Guillaume G   Fogliani Bruno B   Wulff Adrien S AS   L'Huillier Laurent L   Kurata Seikan S   Ueno Saneyoshi S   Isagi Yuji Y   Tsumura Yoshihiko Y   Ito Motomi M  

Applications in plant sciences 20150810 8

<h4>Premise of the study</h4>Microsatellite markers were developed for Callitris sulcata (Cupressaceae), an endangered conifer species in New Caledonia.<h4>Methods and results</h4>Using sequencing by synthesis (SBS) of an RNA-Seq library, 15 polymorphic nuclear and chloroplast microsatellite markers were developed. When evaluated with 48 individuals, these markers showed genetic variations ranging from two to 15 alleles and expected heterozygosity ranging from 0 to 0.881.<h4>Conclusions</h4>Thes  ...[more]

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