Development of nuclear and chloroplast microsatellite markers for the endangered conifer Callitris sulcata (Cupressaceae).

ABSTRACT: Microsatellite markers were developed for Callitris sulcata (Cupressaceae), an endangered conifer species in New Caledonia. Using sequencing by synthesis (SBS) of an RNA-Seq library, 15 polymorphic nuclear and chloroplast microsatellite markers were developed. When evaluated with 48 individuals, these markers showed genetic variations ranging from two to 15 alleles and expected heterozygosity ranging from 0 to 0.881. These markers will be useful for examining the genetic diversity and structure of remaining wild populations and improving the genetic status of ex situ populations.

SUBMITTER: Sakaguchi S 

PROVIDER: S-EPMC4542945 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature