Receptors involved in the modulation of guinea pig urinary bladder motility by prostaglandin D2.

ABSTRACT:

Background and purpose

We have described a urothelium-dependent release of PGD2 -like activity which had inhibitory effects on the motility of guinea pig urinary bladder. Here, we have pharmacologically characterized the receptors involved and localized the sites of PGD2 formation and of its receptors.

Experimental approach

In the presence of selective DP and TP receptor antagonists alone or combined, PGD2 was applied to urothelium-denuded diclofenac-treated urinary bladder strips mounted in organ baths. Antibodies against PGD2 synthase and DP1 receptors were used with Western blots and for histochemistry.

Key results

PGD2 inhibited nerve stimulation -induced contractions in strips of guinea pig urinary bladder with estimated pIC50 of 7.55 ± 0.15 (n = 13), an effect b

SUBMITTER: Guan NN 

PROVIDER: S-EPMC4543610 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature