Protective Polymer Coatings for High-Throughput, High-Purity Cellular Isolation.

ABSTRACT: Cell-based therapies are emerging as the next frontier of medicine, offering a plausible path forward in the treatment of many devastating diseases. Critically, current methods for antigen positive cell sorting lack a high throughput method for delivering ultrahigh purity populations, prohibiting the application of some cell-based therapies to widespread diseases. Here we show the first use of targeted, protective polymer coatings on cells for the high speed enrichment of cells. Individual, antigen-positive cells are coated with a biocompatible hydrogel which protects the cells from a surfactant solution, while uncoated cells are immediately lysed. After lysis, the polymer coating is removed through orthogonal photochemistry, and the isolate has >50% yield of viable cells and these cells p

SUBMITTER: Romero G 

PROVIDER: S-EPMC4544319 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature