Optimized Agrobacterium-mediated sorghum transformation protocol and molecular data of transgenic sorghum plants.

ABSTRACT: Agrobacterium-mediated sorghum transformation frequency has been enhanced significantly via medium optimization using immature embryos from sorghum variety TX430 as the target tissue. The new transformation protocol includes the addition of elevated copper sulfate and 6-benzylaminopurine in the resting and selection media. Using Agrobacterium strain LBA4404, the transformation frequency reached over 10% using either of two different selection marker genes, moPAT or PMI, and any of three different vectors in large-scale transformation experiments. With Agrobacterium strain AGL1, the transformation frequencies were as high as 33%. Using quantitative PCR analyses of 1,182 T₀ transgenic plants representing 675 independent transgenic events, data was colle

SUBMITTER: Wu E 

PROVIDER: S-EPMC4544465 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature