Cold shock induction of recombinant Arctic environmental genes.

ABSTRACT:

Background

Heterologous expression of psychrophilic enzymes in E. coli is particularly challenging due to their intrinsic instability. The low stability is regarded as a consequence of adaptation that allow them to function at low temperatures. Recombinant production presents a significant barrier to their exploitation for commercial applications in industry.

Methods

As part of an enzyme discovery project we have investigated the utility of a cold-shock inducible promoter for low-temperature expression of five diverse genes derived from the metagenomes of marine Arctic sediments. After evaluation of their production, we further optimized for soluble production by building a vector suite from which the environmental genes could be expressed as fusions with solubility tags.

SUBMITTER: Bjerga GE 

PROVIDER: S-EPMC4544801 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature