DNA methylation loci associated with atopy and high serum IgE: a genome-wide application of recursive Random Forest feature selection.

ABSTRACT:

Background

The prevalence of allergic diseases are increasing worldwide, emphasizing the need to elucidate their pathogeneses. The aims of this study were to use a two-stage design to identify DNA methylation levels at cytosine-phosphate-guanine (CpG) sites across the genome associated with atopy and high serum immunoglobulin E (IgE), then to replicate our findings in an independent cohort.

Methods

Atopy was assessed via skin prick tests and high serum IgE. Methylation levels were measured from whole blood using the Illumina Infinium HumanMethylation450 BeadChip from 18-year-old women (n = 245) and men (n = 122) in the Isle of Wight birth cohort. After data cleaning and processing, and removing probes with possible single nucleotide polymorphisms, DNA methylation levels from

SUBMITTER: Everson TM 

PROVIDER: S-EPMC4545869 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature