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De novo transcriptome assembly and analysis of Sf21 cells using illumina paired end sequencing.

ABSTRACT: Spodoptera is an important polyphagous agricultural insect pest in the tropical world. The genomic details are limited to understand the pest biology at molecular level. In the present study, we sequenced and assembled the transcriptome from Sf21 cells into a non redundant set of 24,038 contigs of?~?47.38 Mb in size. A total of 26,390 unigenes were identified from the assembled transcripts and their annotation revealed the prevalent protein domains in Sf21 cells. The present study would provide a resource for gene discovery and development of functional molecular markers to understand the biology of S. frugiperda.

SUBMITTER: Kakumani PK 

PROVIDER: S-EPMC4545970 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

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De novo transcriptome assembly and analysis of Sf21 cells using illumina paired end sequencing.

Kakumani Pavan Kumar PK   Shukla Rohit R   Todur Vivek N VN   Malhotra Pawan P   Mukherjee Sunil K SK   Bhatnagar Raj K RK  

Biology direct 20150820

Spodoptera is an important polyphagous agricultural insect pest in the tropical world. The genomic details are limited to understand the pest biology at molecular level. In the present study, we sequenced and assembled the transcriptome from Sf21 cells into a non redundant set of 24,038 contigs of ~ 47.38 Mb in size. A total of 26,390 unigenes were identified from the assembled transcripts and their annotation revealed the prevalent protein domains in Sf21 cells. The present study would provide  ...[more]

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