Discovery of Novel Plasmodium falciparum Pre-Erythrocytic Antigens for Vaccine Development.

ABSTRACT:

Background

Nearly 100% protection against malaria infection can be achieved in humans by immunization with P. falciparum radiation-attenuated sporozoites (RAS). Although it is thought that protection is mediated by T cell and antibody responses, only a few of the many pre-erythrocytic (sporozoite and liver stage) antigens that are targeted by these responses have been identified.

Methodology

Twenty seven P. falciparum pre-erythrocytic antigens were selected using bioinformatics analysis and expression databases and were expressed in a wheat germ cell-free protein expression system. Recombinant proteins were recognized by plasma from RAS-immunized subjects, and 21 induced detectable antibody responses in mice and rabbit and sera from these immunized animals were used to chara

SUBMITTER: Aguiar JC 

PROVIDER: S-EPMC4546230 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature