Spatial Intensity Distribution Analysis Reveals Abnormal Oligomerization of Proteins in Single Cells.

ABSTRACT: Knowledge of membrane receptor organization is essential for understanding the initial steps in cell signaling and trafficking mechanisms, but quantitative analysis of receptor interactions at the single-cell level and in different cellular compartments has remained highly challenging. To achieve this, we apply a quantitative image analysis technique-spatial intensity distribution analysis (SpIDA)-that can measure fluorescent particle concentrations and oligomerization states within different subcellular compartments in live cells. An important technical challenge faced by fluorescence microscopy-based measurement of oligomerization is the fidelity of receptor labeling. In practice, imperfect labeling biases the distribution of oligomeric states measured within an aggregated system. We ext

SUBMITTER: Godin AG 

PROVIDER: S-EPMC4547338 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature