Structure and function of renal macrophages and dendritic cells from lupus-prone mice.

ABSTRACT:

Objective

To characterize renal macrophages and dendritic cells (DCs) in 2 murine models of lupus nephritis.

Methods

We used a bead-based enrichment step followed by cell sorting to isolate populations of interest from young mice and nephritic mice. Cell morphology was examined by microscopy. Arginase and nitrite production was examined using biochemical assays. The antigen-presenting functions of the cells were determined using mixed lymphocyte reactions. Selected cytokine, chemokine, and Toll-like receptor (TLR) profiles were examined using real-time quantitative polymerase chain reaction.

Results

We identified 2 populations of macrophages and 3 populations of DCs in both of our murine models of lupus (NZB/NZW and [NZW × BXSB]F1 mice). F4/80(high) macrophages, which

SUBMITTER: Sahu R 

PROVIDER: S-EPMC4547797 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature