Differential responses of gut microbiota to the same prebiotic formula in oligotrophic and eutrophic batch fermentation systems.

ABSTRACT: The same prebiotics have produced inconsistent effects on microbiota when evaluated in different batch fermentation studies. To understand the reasons behind these discrepancies, we compared impact of one prebiotic formula on the same inoculated fecal microbiota in two frequently used batch systems: phosphate-buffered saline (PBS, oligotrophic) and basal culture medium (BCM, eutrophic). The microbiota was monitored using 454 pyrosequencing. Negative controls (no prebiotic) of both systems showed significant shifts in the microbiota during fermentation, although their pH remained relatively stable, especially in BCM, with increases in Bilophila and Escherichia/Shigella but a decrease in Faecalibacterium. We identified prebiotic responders via redundancy analysis by including both baseline a

SUBMITTER: Long W 

PROVIDER: S-EPMC4548253 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature