Proteomic profiling of aging in glomeruli of mice by using two-dimensional differential gel electrophoresis.

ABSTRACT:

Background

Glomerular proteins were analyzed by proteomics to screen proteins participating in maturation of glomeruli before senescence and to find key proteins involved in the aging process.

Material and methods

Glomeruli of C57BL/6 mice at 8 and 20 weeks were separated by kidney perfusion. Proteomic profiles of glomeruli were investigated by using two-dimensional differential gel electrophoresis and MALDI-TOF mass spectrometry.

Results

We identified 22 differentially expressed proteins. Among them, 3 proteins were significantly up-regulated and 19 proteins were significantly down-regulated in mature mice. Out of these 22 proteins, 18% take part in protein transport, protein targeting, and proteolysis; 5% in glycolysis; 14% in transcription; 9% in electron transport

SUBMITTER: Liu X 

PROVIDER: S-EPMC4548697 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature