Mass Spectrometric Identification and Differentiation of Botulinum Neurotoxins through Toxin Proteomics.

ABSTRACT: Botulinum neurotoxins (BoNTs) cause the disease botulism, which can be lethal if untreated. There are seven known serotypes of BoNT, A-G, defined by their response to antisera. Many serotypes are distinguished into differing subtypes based on amino acid sequence and immunogenic properties, and some subtypes are further differentiated into toxin variants. Toxin characterization is important as different types of BoNT can respond differently to medical countermeasures for botulism, and characterization of the toxin can aid in epidemiologic and forensic investigations. Proteomic techniques have been established to determine the serotype, subtype, or toxin variant of BoNT. These techniques involve digestion of the toxin into peptides, tandem mass spectrometric (MS/MS) analysis of the peptides,

SUBMITTER: Kalb SR 

PROVIDER: S-EPMC4549225 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature