Transposase interaction with the β sliding clamp: effects on insertion sequence proliferation and transposition rate.

ABSTRACT: Insertion sequences (ISs) are ubiquitous and abundant mobile genetic elements in prokaryotic genomes. ISs often encode only one protein, the transposase, which catalyzes their transposition. Recent studies have shown that transposases of many different IS families interact with the β sliding clamp, a DNA replication factor of the host. However, it was unclear to what extent this interaction limits or favors the ability of ISs to colonize a chromosome from a phylogenetically-distant organism, or if the strength of this interaction affects the transposition rate. Here we describe the proliferation of a member of the IS1634 family in Acidiphilium over ~600 generations of cultured growth. We demonstrate that the purified transposase binds to the β sliding clamp of Acidiphilium, Leptospirillum

SUBMITTER: Diaz-Maldonado H 

PROVIDER: S-EPMC4549789 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature