The Escherichia coli NarL receiver domain regulates transcription through promoter specific functions.

ABSTRACT:

Background

The Escherichia coli response regulator NarL controls transcription of genes involved in nitrate respiration during anaerobiosis. NarL consists of two domains joined by a linker that wraps around the interdomain interface. Phosphorylation of the NarL N-terminal receiver domain (RD) releases the, otherwise sequestered, C-terminal output domain (OD) that subsequently binds specific DNA promoter sites to repress or activate gene expression. The aim of this study is to investigate the extent to which the NarL OD and RD function independently to regulate transcription, and the affect of the linker on OD function.

Results

NarL OD constructs containing different linker segments were examined for their ability to repress frdA-lacZ or activate narG-lacZ reporter fusion gen

SUBMITTER: Katsir G 

PROVIDER: S-EPMC4549865 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature