Dynamic distribution and the role of abscisic acid during seed development of a lady's slipper orchid, Cypripedium formosanum.

ABSTRACT:

Background and aims

Although abscisic acid (ABA) is commonly recognized as a primary cause of seed dormancy, there is a lack of information on the role of ABA during orchid seed development. In order to address this issue, the localization and quantification of ABA were determined in developing seeds of Cypripedium formosanum.

Methods

The endogenous ABA profile of seeds was measured by enzyme-linked immunosorbent assay (ELISA). Temporal and spatial distributions of ABA in developing seeds were visualized by immunohistochemical staining with monoclonal ABA antibodies. Fluoridone was applied to test the causal relationship between ABA content and seed germinability.

Key results

ABA content was low at the proembryo stage, then increased rapidly from 120 to 150 days after

SUBMITTER: Lee YI 

PROVIDER: S-EPMC4549955 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature