Accelerated Optical Projection Tomography Applied to In Vivo Imaging of Zebrafish.

ABSTRACT: Optical projection tomography (OPT) provides a non-invasive 3-D imaging modality that can be applied to longitudinal studies of live disease models, including in zebrafish. Current limitations include the requirement of a minimum number of angular projections for reconstruction of reasonable OPT images using filtered back projection (FBP), which is typically several hundred, leading to acquisition times of several minutes. It is highly desirable to decrease the number of required angular projections to decrease both the total acquisition time and the light dose to the sample. This is particularly important to enable longitudinal studies, which involve measurements of the same fish at different time points. In this work, we demonstrate that the use of an iterative algorithm to reconstruct s

SUBMITTER: Correia T 

PROVIDER: S-EPMC4550250 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature