Feasibility of Cowpea chlorotic mottle virus-like particles as scaffold for epitope presentations.

ABSTRACT:

Background & methods

Within the last decade Virus-Like Particles (VLPs) have increasingly received attention from scientists for their use as a carrier of (peptide) molecules or as scaffold to present epitopes for use in subunit vaccines. To test the feasibility of Cowpea chlorotic mottle virus (CCMV) particles as a scaffold for epitope presentation and identify sites for epitope fusion or insertion that would not interfere with virus-like-particle formation, chimeric CCMV coat protein (CP) gene constructs were engineered, followed by expression in E. coli and assessment of VLP formation. Various constructs were made encoding a 6x-His-tag, or selected epitopes from Influenza A virus [IAV] (M2e, HA) or Foot and Mouth Disease Virus [FMDV] (VP1 and 2C). The epitopes were either insert

SUBMITTER: Hassani-Mehraban A 

PROVIDER: S-EPMC4551372 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature