A Cell-Permeable ATP Analogue for Kinase-Catalyzed Biotinylation.

ABSTRACT: ATP analogues have been powerful compounds for the study of kinase-catalyzed phosphorylation. However, the cell impermeability of ATP analogues has largely limited their use to in vitro lysate-based experiments. Herein, we report the first cell-permeable ATP analogue, ATP-polyamine-biotin (APB). APB is shown to promote biotin labeling of kinase substrates in live cells and has future applications in phosphoprotein purification and analysis. More generally, these studies provide a foundation for the development of additional cell-permeable ATP analogues for cell-signaling research.

SUBMITTER: Fouda AE 

PROVIDER: S-EPMC4551444 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature