A simple procedure for directly obtaining haplotype sequences of diploid genomes.

ABSTRACT:

Background

Almost all genome sequencing projects neglect the fact that diploid organisms contain two genome copies and consequently what is published is a composite of the two. This means that the relationship between alternate alleles at two or more linked loci is lost. We have developed a simplified method of directly obtaining the haploid sequences of each genome copy from an individual organism.

Results

The diploid sequences of three groups of cattle samples were obtained using a simple sample preparation procedure requiring only a microscope and a haemocytometer. Samples were: 1) lymphocytes from a single Angus steer; 2) sperm cells from an Angus bull; 3) lymphocytes from East African Zebu (EAZ) cattle collected and processed in a field laboratory in Eastern Kenya. Hapl

SUBMITTER: Noyes HA 

PROVIDER: S-EPMC4551521 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature