The effects of human Wharton's jelly cell transplantation on the intervertebral disc in a canine disc degeneration model.

ABSTRACT:

Introduction

Cell-based therapy was a promising treatment method for disc degenerative diseases. Wharton's jelly cell (WJC) has been explored to cure various human diseases, while it still remains unknown about this MSC for disc repair. In our prior work, WJCs could differentiate into nucleus pulposus (NP)-like cells by co-culturing with NP cells in vitro. Thence, the aim of this study was further to investigate the survival and function of WJCs in vivo after transplantation into degenerated canine discs.

Method

WJCs were isolated from human umbilical cords and labeled with EGFP. The degeneration of L4-5, L5-6, and L6-7 discs of beagles was induced by aspirating the NP tissues. Four weeks after the operation, the injured discs were left to be no treatment at L4-5 (DS group),

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC4551525 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature