RNA sequencing from human neutrophils reveals distinct transcriptional differences associated with chronic inflammatory states.

ABSTRACT:

Background

The transcriptional complexity of mammalian cells suggests that they have broad abilities to respond to specific environmental stimuli and physiologic contexts. These abilities were not apparent a priori from the structure of mammalian genomes, but have been identified through detailed transcriptome analyses. In this study, we examined the transcriptomes of cells of the innate immune system, human neutrophils, using RNA sequencing (RNAseq).

Methods

We sequenced poly-A RNA from nine individual samples corresponding to specific phenotypes: three children with active, untreated juvenile idiopathic arthritis (JIA)(AD), three children with the same disease whose disease was inactive on medication (CRM), and three children with cystic fibrosis (CF).

Results

We de

SUBMITTER: Jiang K 

PROVIDER: S-EPMC4551565 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature