Transcriptome profiling during a natural host-parasite interaction.

ABSTRACT:

Background

Infection outcome in some coevolving host-pathogens is characterised by host-pathogen genetic interactions, where particular host genotypes are susceptible only to a subset of pathogen genotypes. To identify candidate genes responsible for the infection status of the host, we exposed a Daphnia magna host genotype to two bacterial strains of Pasteuria ramosa, one of which results in infection, while the other does not. At three time points (four, eight and 12 h) post pathogen exposure, we sequenced the complete transcriptome of the hosts using RNA-Seq (Illumina).

Results

We observed a rapid and transient response to pathogen treatment. Specifically, at the four-hour time point, eight genes were differentially expressed. At the eight-hour time point, a single gene w

SUBMITTER: McTaggart SJ 

PROVIDER: S-EPMC4551569 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature