Mediator tail subunits can form amyloid-like aggregates in vivo and affect stress response in yeast.

ABSTRACT: The Med2, Med3 and Med15 proteins form a heterotrimeric subdomain in the budding yeast Mediator complex. This Med15 module is an important target for many gene specific transcription activators. A previous proteome wide screen in yeast identified Med3 as a protein with priogenic potential. In the present work, we have extended this observation and demonstrate that both Med3 and Med15 form amyloid-like protein aggregates under H2O2 stress conditions. Amyloid formation can also be stimulated by overexpression of Med3 or of a glutamine-rich domain present in Med15, which in turn leads to loss of the entire Med15 module from Mediator and a change in stress response. In combination with genome wide transcription analysis, our data demonstrate that amyloid formation can change the subunit compos

SUBMITTER: Zhu X 

PROVIDER: S-EPMC4551914 | biostudies-literature | 2015 Sep

REPOSITORIES: biostudies-literature