Prediction of Functionally Important Phospho-Regulatory Events in Xenopus laevis Oocytes.

ABSTRACT: The African clawed frog Xenopus laevis is an important model organism for studies in developmental and cell biology, including cell-signaling. However, our knowledge of X. laevis protein post-translational modifications remains scarce. Here, we used a mass spectrometry-based approach to survey the phosphoproteome of this species, compiling a list of 2636 phosphosites. We used structural information and phosphoproteomic data for 13 other species in order to predict functionally important phospho-regulatory events. We found that the degree of conservation of phosphosites across species is predictive of sites with known molecular function. In addition, we predicted kinase-protein interactions for a set of cell-cycle kinases across all species. The degree of conservation of kinase-protein inte

SUBMITTER: Johnson JR 

PROVIDER: S-EPMC4552029 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature