Intracellular delivery of fluorescent protein into viable wheat microspores using cationic peptides.

ABSTRACT: Microspores are specialized generative cells with haploid genome that demonstrate the amenability toward embryogenesis under certain conditions. The induced microspore culture technique is largely exploited by the breeding programs of wheat and other crops due to its high efficiency for generation of the large number of haploid plants in the relatively short period of time. The ability to produce mature double haploid plant from a single cell has also attracted attention of the plant biotechnologists in the past few years. More importantly, the possibility to deliver proteins for improvement of embryogenesis and the genome modification purposes holds great potential for transgene-free wheat biotechnology. In the present study, we examined the ability of cationic and amphipathic cell penetr

SUBMITTER: Bilichak A 

PROVIDER: S-EPMC4552043 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature