Measuring Binding Kinetics of Antibody-Conjugated Gold Nanoparticles with Intact Cells.

ABSTRACT: Antibody-conjugated nanomaterials have attracted much attention because of their applications in nanomedicine and nanotheranostics, and amplification of detection signals. For many of these applications, the nanoconjugates must bind with a cell membrane receptor (antigen) specifically before entering the cells and reaching the final target, which is thus important but not well understood. Here, a plasmonic imaging study of the binding kinetics of antibody-conjugated gold nanoparticles with antigen-expressing cells is presented, and the results are compared with that of the nanoparticle-free antibody. It is found that the nanoconjugates can significantly affect the binding kinetics compared with free antibody molecules, depending on the density of the antibody conjugated on the nanoparticle

SUBMITTER: Yin L 

PROVIDER: S-EPMC4552349 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature